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Details

Role Group Leader / Principal Research Fellow
Research area Stem Cell Medicine

Contact

Available for student supervision
Dr Elizabeth Ng is a Team Leader in the Blood Development Laboratory at the Murdoch Children's Research Institute (MCRI). After completing her undergraduate degree at the University of Melbourne, she received her PhD from Monash University in 2013. A major focus of her work is to generate transplantable haematopoietic stem cells (HSCs) from human pluripotent stem cells for tissue repair and regeneration, disease modeling and the testing of pharmaceuticals and other therapeutic products. Dr Ng has devised and patented protocols for haematopoietic differentiation together with a differentiation medium that supports the efficient production of large numbers of blood cells. APEL medium has been licensed and commercialised by STEMCELL Technologies. She has maintained a long-standing collaboration with Professor Andrew Elefanty (Blood Development) and Professor Ed Stanley (Immune Development), together making important contributions to the development of human pluripotent stem cell as a powerful experimental platform to drive advancement in differentiation systems, cell culture medium formulation and the use of genetic modifications of human pluripotent stem cell lines.

Her work has identified that the expression of the HOXA genes during differentiation is crucial for generating haematopoietic cells with a transcriptional profile similar to the 'preHSCs' seen in the aorta-gonad mesonephros (AGM) region of the human embryo. Her current work focuses upon the challenge of maturing these cells further to produce engraftable HSCs and to develop in vitro 'niches' that mimic the bone marrow in order to sustain them. Her work is supported by grants from the NHMRC, a research collaboration with CSL Ltd and she is currently a Principal Investigator of the Novo Nordisk Foundation Center for Stem Cell Medicine.

Dr Ng holds active collaborations with:
• Prof Andrew Elefanty, Blood Development Laboratory, Murdoch Children's Research Institute
• Prof Ed Stanley, Immune Development Laboratory, Murdoch Children's Research Institute
• Prof Constanze Bonifer, Institute of Cancer and Genomic Sciences, University of Birmingham
• Prof Hanna Mikkola, Department of Molecular, Cell and Developmental Biology University of California
Dr Elizabeth Ng is a Team Leader in the Blood Development Laboratory at the Murdoch Children's Research Institute (MCRI). After completing her undergraduate degree at the University of Melbourne, she received her PhD from Monash University in 2013. A...
Dr Elizabeth Ng is a Team Leader in the Blood Development Laboratory at the Murdoch Children's Research Institute (MCRI). After completing her undergraduate degree at the University of Melbourne, she received her PhD from Monash University in 2013. A major focus of her work is to generate transplantable haematopoietic stem cells (HSCs) from human pluripotent stem cells for tissue repair and regeneration, disease modeling and the testing of pharmaceuticals and other therapeutic products. Dr Ng has devised and patented protocols for haematopoietic differentiation together with a differentiation medium that supports the efficient production of large numbers of blood cells. APEL medium has been licensed and commercialised by STEMCELL Technologies. She has maintained a long-standing collaboration with Professor Andrew Elefanty (Blood Development) and Professor Ed Stanley (Immune Development), together making important contributions to the development of human pluripotent stem cell as a powerful experimental platform to drive advancement in differentiation systems, cell culture medium formulation and the use of genetic modifications of human pluripotent stem cell lines.

Her work has identified that the expression of the HOXA genes during differentiation is crucial for generating haematopoietic cells with a transcriptional profile similar to the 'preHSCs' seen in the aorta-gonad mesonephros (AGM) region of the human embryo. Her current work focuses upon the challenge of maturing these cells further to produce engraftable HSCs and to develop in vitro 'niches' that mimic the bone marrow in order to sustain them. Her work is supported by grants from the NHMRC, a research collaboration with CSL Ltd and she is currently a Principal Investigator of the Novo Nordisk Foundation Center for Stem Cell Medicine.

Dr Ng holds active collaborations with:
• Prof Andrew Elefanty, Blood Development Laboratory, Murdoch Children's Research Institute
• Prof Ed Stanley, Immune Development Laboratory, Murdoch Children's Research Institute
• Prof Constanze Bonifer, Institute of Cancer and Genomic Sciences, University of Birmingham
• Prof Hanna Mikkola, Department of Molecular, Cell and Developmental Biology University of California

Top Publications

  • Ng, ES, Davis, R, Stanley, EG, Elefanty, AG. A protocol describing the use of a recombinant protein-based, animal product-free medium (APEL) for human embryonic stem cell differentiation as spin embryoid bodies.. Nat Protoc 3(5) : 768 -776 2008
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  • Ng, ES, Davis, RP, Hatzistavrou, T, Stanley, EG, Elefanty, AG. Directed differentiation of human embryonic stem cells as spin embryoid bodies and a description of the hematopoietic blast colony forming assay.. Curr Protoc Stem Cell Biol Chapter 1: Unit -1D.3 2008
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  • Hirst, CE, Ng, ES, Azzola, L, Voss, AK, Thomas, T, Stanley, EG, Elefanty, AG. Transcriptional profiling of mouse and human ES cells identifies SLAIN1, a novel stem cell gene.. Dev Biol 293(1) : 90 -103 2006
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  • Mossman, AK, Sourris, K, Ng, E, Stanley, EG, Elefanty, AG. Mixl1 and oct4 proteins are transiently co-expressed in differentiating mouse and human embryonic stem cells.. Stem Cells Dev 14(6) : 656 -663 2005
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  • Ng, ES, Davis, RP, Azzola, L, Stanley, EG, Elefanty, AG. Forced aggregation of defined numbers of human embryonic stem cells into embryoid bodies fosters robust, reproducible hematopoietic differentiation.. Blood 106(5) : 1601 -1603 2005
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