You are here
Karena Last is a Research Assistant with more than twenty years of experience in Cartilage Biology research. Initially she worked with Associate Professor Amanda Fosang in Department of Medicine, University of Melbourne, and since 1994 in the Department of Paediatrics. Karena has been a Research Associate with Murdoch Childrens Research Institute since its inception in 2000.
- Research Assistant, University of Melbourne, Department of Paediatrics
The Arthritis Research Group studies the molecular events that lead to cartilage, bone and joint destruction in arthritis, focusing primarily on molecules that define cartilage structure, and the enzymes that destroy them. Although there are common patterns of cartilage destruction across all arthritic conditions, there are also specific points of difference at the molecular level. The group focuses on defining anabolic and catabolic pathways in juvenile arthritis and osteoarthritis (the most common form of arthritis).
- How aggrecanases contribute to cartilage destruction
- Mice resistant to collagen destruction teach us about skeletal development
- Signalling through a bioactive fragment of aggrecan
- An ultrasensitive assay to detect aggrecan degradation products in patient fluids
Stanton, H., Rogerson, FM., East, CJ., Golub, SB., Lawlor, KE., Meeker, CT, Little, CB., Last, K., Farmer, PJ., Campbell, IK., Fourie, AM., & Fosang, AJ. (2005) ADAMTS-5 is the major aggrecanase in mouse cartilage, in vivo and in vitro. Nature 434, 648-652
Struglics, A., Lohmander, S., Last K., Akikusa J., Allen R. & Fosang, AJ. (2012) Aggrecanase cleavage in juvenile idiopathic arthritis patients is minimally detected in the aggrecan interglobular domain but robust at the aggrecan C-terminus. Arthritis Rheum 64, 4151-4161
Bastow ER., Last K., Golub, S., Stow J., Stanley A. & Fosang AJ. (2012) Evidence for lysosomal exocytosis and release of aggrecan-degrading hydrolases from hypertrophic chondrocytes, in vitro and in vivo. Biology Open 1, 318-328
Stanton, H., Golub, SB., Rogerson, FM., Last, K., Little, CB., Fosang, AJ. (2011) Investigating ADAMTS-mediated aggrecanolysis in mouse cartilage. Nature Protocols 6, 388-404
Rogerson, FM., Chung, YM., Deutscher, ME., Last, K. & Fosang AJ. (2010) Cytokine-induced increases in ADAMTS-4 mRNA expression do not lead to increased aggrecanase activity in ADAMTS-5-deficient mice. Arthritis Rheum. 62, 3365-3373
Fosang, AJ., Last, K., Poon, CJ., & Plaas, AH. (2009) Keratan sulphate in the interglobular domain has a microstructure that is distinct from keratan sulphate elsewhere on pig aggrecan. Matrix Biol. 28, 53-61