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Professor Ed Stanley
Professor Ed Stanley completed his PhD at the Ludwig Institute for Cancer Research in Melbourne, working on hematopoietic growth factors and gene targeting in mouse embryonic stem cells. Following his PhD, Professor Stanley undertook post-doctoral studies in Developmental Biology at the National Institute for Medical Research, Mill Hill, London. On his return to Australia, he joined Richard Harvey's cardiac developmental biology laboratory at the Walter and Eliza Hall Institute, Melbourne. Together with colleague Professor Andrew Elefanty, he moved to Monash University in 2002 and established The Embryonic Stem Cell Differentiation Laboratory.
The laboratory focused on human embryonic stem cell (ESC) differentiation along mesodermal (blood, endothelium and heart) and endodermal (pancreas) lineages. This group developed novel methods for genetic modification of pluripotent stem cells and serum free differentiation protocols. In 2012, Professor Stanley to moved the Murdoch Childrens Research Institute, where he currently heads the Stem Cell Technology Laboratory. He is an NHMRC senior research fellow.
- Honorary Professor, Department of Paediatrics, University of Melbourne
- Adjunct Professor,Department of Anatomy and Developmental Biology, Monash University
- 1992-93: Human Frontiers in Science Program Organization long-term fellowship (London)
- 1993- 1996: C.J. Martin Fellowship, National Institute for Medical Research (London, UK)
- 1996- 1998: C.J. Martin Fellowship, Walter & Eliza Hall Institute (Australia)
- 2010- 2014: NHMRC Research Fellowship (SRFA)
- 2015- 2019: NHMRC Research Fellowship (SRFA)
A major goal of Professor Stanley's current work involves using stem cell differentiation systems to understand human development, to generate tools for drug discovery, and to provide a source of cells for future medical applications. His group has a special interest in stem cell derived insulin producing beta cells, their generation and function. In addition, he has continuing interest in devising new methods for genetically modifying pluripotent stem cells, with the goal of improving the versatility of this system as an accessible experimental platform. This includes the generation of expression systems that enable the inducible regulation of introduced genes and methods for knocking out endogenous genes.
- Genetic manipulation of pluripotent stem cells
- Analysis of stem cell derived beta cells
- Making blood cells in the laboratory
- Induced pluripotent stem cell models of human disease
- Stem cell models of human development
FOXN1 (GFP/w) Reporter hESCs Enable Identification of Integrin-ß4, HLA-DR, and EpCAM as Markers of Human PSC-Derived FOXN1(+) Thymic Epithelial Progenitors. Soh CL, Giudice A, Jenny RA, Elliott DA, Hatzistavrou T, Micallef SJ, Kianizad K, Seach N, Zúñiga-Pflücker JC, Chidgey AP, Trounson A, Nilsson SK, Haylock DN, Boyd RL, Elefanty AG, Stanley EG. Stem Cell Reports. 2014 May 22;2(6):925-37. doi: 10.1016/j.stemcr.2014.04.009. eCollection 2014 Jun 3. PMID: 24936476 [PubMed]
Efficient endoderm induction from human pluripotent stem cells by logically directing signals controlling lineage bifurcations. Loh KM, Ang LT, Zhang J, Kumar V, Ang J, Auyeong JQ, Lee KL, Choo SH, Lim CY, Nichane M, Tan J, Noghabi MS, Azzola L, Ng ES, Durruthy-Durruthy J, Sebastiano V, Poellinger L, Elefanty AG, Stanley EG, Chen Q, Prabhakar S, Weissman IL, Lim B. Cell Stem Cell. 2014 Feb 6;14(2):237-52. doi: 10.1016/j.stem.2013.12.007. Epub 2014 Jan 9. PMID: 24412311
Directing human embryonic stem cell differentiation towards a renal lineage generates a self-organizing kidney. Takasato M, Er PX, Becroft M, Vanslambrouck JM, Stanley EG, Elefanty AG, Little MH. Nat Cell Biol. 2014 Jan;16(1):118-26. PMID: 24335651
Rewiring Immunity: Generating a Functional Thymus from hESCs… Are We There Yet? Boyd RL, Soh CL, Boyd NR, Stanley EG, Chidgey AP. Cell Stem Cell. 2013 Aug 1;13(2):135-6. PMID: 23910077
WNT3A Promotes Hematopoietic or Mesenchymal Differentiation from hESCs Depending on the Time of Exposure. K Gertow, CE Hirst, QC Yu, ES Ng, LA Pereira, RP Davis, EG Stanley, AG Elefanty Stem Cell Reports 2013. 1 (1), 53-65.
Functional maturation of hPSC-derived forebrain interneurons requires an extended timeline and mimics human neural development. Nicholas CR, Chen J, Tang Y, Southwell DG, Chalmers N, Vogt D, Arnold CM, Chen YJ, Stanley EG, Elefanty AG, Sasai Y, Alvarez-Buylla A, Rubenstein JL, Kriegstein AR. Cell Stem Cell. 2013 May 2;12(5):573-86. PMID: 23642366
APELIN promotes hematopoiesis from human embryonic stem cells. Yu QC, Hirst CE, Costa M, Ng ES, Schiesser JV, Gertow K, Stanley EG, Elefanty AG. Blood. 2012 Jun 28;119(26):6243-54
INS(GFP/w) human embryonic stem cells facilitate isolation of in vitro derived insulin-producing cells. Micallef SJ, Li X, Schiesser JV, Hirst CE, Yu QC, Lim SM, Nostro MC, Elliott DA, Sarangi F, Harrison LC, Keller G, Elefanty AG, Stanley EG. Diabetologia. 2012 Mar;55(3):694-706. PMID: 22120512 Free PMC Article
NKX2-5eGFP/w hESCs for isolation of human cardiac progenitors and cardiomyocytes. Elliott DA, Braam SR, Koutsis K, Ng ES, Jenny R, Lagerqvist EL, Biben C, Hatzistavrou T, Hirst CE, Yu QC, Skelton RJ, Ward-van Oostwaard D, Lim SM, Khammy O, Li X, Hawes SM, Davis RP, Goulburn AL, Passier R, Prall OW, Haynes JM, Pouton CW, Kaye DM, Mummery CL, Elefanty AG*, Stanley EG*. Nat Methods. 2011 Oct 23;8(12):1037-40. PMID: 22020065 *Equal contributors
A targeted NKX2.1 human embryonic stem cell reporter line enables identification of human basal forebrain derivatives. Goulburn AL, Alden D, Davis RP, Micallef SJ, Ng ES, Yu QC, Lim SM, Soh CL, Elliott DA, Hatzistavrou T, Bourke J, Watmuff B, Lang RJ, Haynes JM, Pouton CW, Giudice A, Trounson AO, Anderson SA, Stanley EG, Elefanty AG. Stem Cells. 2011 Mar;29(3):462-73. PMID: 21425409