Dr Sara Howden
Dr Howden completed a BSc at the University of Melbourne in 2003, and joined the MCRI as an honours student. She received an "Australian Postgraduate Award" and completed her PhD in 2008 at the MCRI after working extensively with bacterial artificial chromosomes, including genetic manipulation and large-scale purification of, and effective introduction and targeted integration into human cells. In 2008 Dr Howden was offered a postdoctoral position in the laboratory of stem cell pioneer, Dr James Thomson, at the University of Wisconsin (Madison, USA). This was supported by an Overseas Biomedical (CJ Martin) Fellowship. During her postdoctoral years Dr Howden gained vast expertise with regards to pluripotent stem cell derivation, culture and genetic modification and was first to describe targeted gene repair of patient-specific iPS cells. She has extensive experience with the CRISPR/Cas9 system and has recently developed a one-step protocol that permits rapid and efficient derivation of gene-edited iPS cells from human fibroblasts. After returning to the Australia, she joined the laboratory of CIA Little in 2016 as a Senior Research Officer and has a joint role as director of the MCRI Gene Editing core facility.
- Honorary Appointment, Department of Paediatrics, University of Melbourne
NHRMC Overseas Biomedical (CJ Martin) Fellowship
Generation of reporter lines to facilitate directed differentiation of kidney cells from human pluripotent stem cells
Howden SE, McColl B, Glaser A, Vadolas J, Petrou S, Little MH, Elefanty AG, Stanley EG. (2016) A Cas9 Variant for Efficient Generation of Indel-Free Knockin or Gene-Corrected Human Pluripotent Stem Cells. Stem Cell Reports 7:508-17
Kao T, Labonne T, Niclis JC, Chaurasia R, Lokmic Z, Qian E, Bruveris FF, Howden SE, Motazedian A, Schiesser JV, Costa M, Sourris K, Ng E, Anderson D, Giudice A, Farlie P, Cheung M, Lamande SR, Penington AJ, Parish CL, Thomson LH, Rafii A, Elliott DA, Elefanty AG, Stanley EG. (2016) GAPTrap: A Simple Expression System for Pluripotent Stem Cells and Their Derivatives. Stem Cell Reports 7:518-26.
Phelan DG, Anderson DJ, Howden SE, Wong RC, Hickey PF, Pope K, Wilson GR, Pébay A, Davis AM, Petrou S, Elefanty AG, Stanley EG, James PA, Macciocca I, Bahlo M, Cheung MM, Amor DJ, Elliott DA, Lockhart PJ. (2016) ALPK3-deficient cardiomyocytes generated from patient-derived induced pluripotent stem cells and mutant human embryonic stem cells display abnormal calcium handling and establish that ALPK3 deficiency underlies familial cardiomyopathy. Eur Heart J 37:2586-90
Howden SE, Maufort JP, Duffin B, Elefanty AG, Stanley EG, Thomson JA. (2015) Simultaneous reprogramming and gene correction of patient fibroblasts. Stem Cell Reports 5:1109-18
Capowski EE, Simonett JM, Clark EM, Wright LS, Howden SE, Wallace KA, Petelinsek AM, Pinilla I, Phillips MJ, Meyer JS, Schneider BL, Thomson JA, Gamm DM. (2014) Loss of MITF expression during human embryonic stem cell differentiation disrupts retinal pigment epithelium development and optic vesicle cell proliferation. Hum Mol Genet 23: 6332-44
Hou Z, Zhang Y, Propson NE, Howden SE, Chu LF, Sontheimer EJ, Thomson JA. (2013) Efficient genome engineering in human pluripotent stem cells using Cas9 from Neisseria meningitidis. Proc Natl Acad Sci USA 110:15644-9
Brumbaugh J, Hou Z, Russell JD, Howden SE, Yu P, Ledvina AR, Coon JJ, Thomson JA. (2012) Phosphorylation regulates human OCT4. Proc Natl Acad Sci USA 109:7162-8
Meyer JS, Howden SE, Wallace KA, Verhoeven AD, Wright LS, Capowski EE, Pinilla I, Martin JM, Tian S, Stewart R, Pattnaik B, Thomson J, Gamm DM. (2011) Optic Vesicle-like Structures Derived from Human Pluripotent Stem Cells Facilitate a Customized Approach to Retinal Disease Treatment. Stem Cells 29:1206-18
Chen G, Gulbranson DR, Hou Z, Bolin JM, Ruotti V, Probasco MD, Smuga-Otto K, Howden SE, Diol NR, Propson NE, Wagner R, Lee GO, Antosiewicz-Bourget J, Teng JM, Thomson JA. (2011) Chemically defined conditions for human iPSC derivation and culture. Nat Methods 8:424-9 (citations: 362)
Howden SE, Gore A, Li Z, Fung HL, Nisler BS, Nie J, Chen G, McIntosh BE, Gulbranson DR, Diol NR, Taapken SM, Vereide DT, Montgomery KD, Zhang K, Gamm DM, Thomson JA. (2011) Genetic correction and analysis of induced pluripotent stem cells from a patient with gyrate atrophy. Proc Natl Acad Sci USA 108:6537-42
Zaibak F, Kozlovski J, Vadolas J, Sarsero JP, Williamson R, Howden SE. (2009) Integration of functional bacterial artificial chromosomes into human cord blood-derived multipotent stem cells. Gene Ther 6:404-14
Howden SE, Voullaire L, Wardan H, Williamson R, Vadolas J. (2008) Site-specific, Rep-mediated integration of the intact beta-globin locus in the human erythroleukaemic cell line K562. Gene Ther 15:1372-83 ?
Howden SE, Voullaire L, Vadolas J. (2008) The transient expression of mRNA coding for Rep protein from AAV facilitates targeted plasmid integration. J Gene Med 10:42-50
Howden SE, Wardan H, Voullaire L, McLenachan S, Williamson R, Ioannou P, Vadolas J. (2006) Chromatin-binding regions of EBNA1 protein facilitate the enhanced transfection of Epstein-Barr virus-based vectors. Hum Gene Ther 17:833-44 ?