You are here
Dr Ruili Li
Dr Ruili Li (BS, MS, Ph.D) is a senior research fellow at the Surgical Research group, Murdoch Children's Research Institute, Honorary senior research fellow at the Paediatric Department of Melbourne University, Australia.
Dr Li started her research in reproductive biology when she did her Master's degree at the China Agriculture University on cryopreservation of rabbit embryos. She then came to Australia and completed her Ph.D in cryopreservation of mouse, goat and sheep embryos and embryonic cell development on the attempt to isolate embryonic stem cells under the supervision of Prof. Alan Trounson and Prof. Euan Roberts.
Dr Li joined Prof Richard Harvey's lab at the Water and Eliza Hall Institute after her Ph.D completion and focused on the establishment of the gene knockout system using embryonic stem cells followed by many years' experience of generating and analysis of genetically modified mice. She gained lots of experience on molecular biology, developmental biology and haematology while she was working side-by-side with many experts there.
Dr Li joined Prof Andrew Sinclair's Lab at Murdoch Children's Research Institute in 2005 to study germ cell development during sex determination which involved in generating transgenic mice with inducible gene expression (TCCre, TCGFP, MkxHA mice) using ES cells and tetraploid embryos as well as blastocyst injection. With Prof Sinclair and MCRI executive committee's support, Dr Li involved in the establishment of MCRI facility of the transgenic and embryo service by drafting the business plan and was subsequently appointed as the manager of the facility during 2008-2010. Dr Li joined the Surgical Research group in 2010 and currently leads the laboratory research on the postnatal germ cell development and testicular descent using various transgenic, gene-knockout and naturally mutated rodent models as well as human biopsies of undescended testis (UDT).
- Associate Department of Paediatrics, University of Melbourne
Postgraduate scholarship for Ph.D from University of New South Wales, Australia
Dr Li has worked on testicular development for more than 10 years since she joined Prof Andrew Sinclair's Lab at Murdoch Children's Research Institute in 2005, after postdoctorial experience on developmental cardiology and developmental haematology using gene-knockout technology at the Water and Eliza Hall Institute.
Dr Li joined the surgical research group in 2010 and currently leads the laboratory research on postnatal germ cell development of the testis and testicular descent using various transgenic, gene-knockout and naturally mutated rodent models as well as human biopsies of undescended testis.
Congenital undescended testis (UDT), or cryptorchidism is the most common abnormality in newborn boys, affects 2-4% of baby boys and leads to a 5-10 fold increase in testicular cancer (seminoma), and 30-60% risk of infertility later in life. Seminomas in young men with UDT arise from premalignant carcinoma-in-situ (CIS) cells which were thought to be mutant fetal germ cells. However, studies from Dr Li's team and others have demonstrated that early postnatal germ cell development is abnormal in UDT due to the interruption of minipuberty at 3-6 months of age. The aim of Dr Li's research projects is to better understand the biology related to the problems of cryptorchidism with infertility and testicular cancer to provide evidence-based guidance for surgery on UDT to prevent these problems.
Dr Li is also involved in the research on the duodenum development led by Dr Warwick Teague since beginning of 2015 to 2018 with her expertise on the analysis of gene knockout mice.
Postnatal germ cell development of the testis
Burton E, Abeydeera SA, Sarila G, Cho H-J, Wu S, Tien MY, Hutson J, Li R (2020) The role of gonadotrophins in gonocyte transformation during minipuberty. Pediatr. Surg. Int. 36 (11): 1379-1385
Li R, Wu S, Kaur M, Zong S, Abeydeera SA, Bao T, Arif S, Hutson JM (2020) Bcl2-Associated X (BAX) Knockout in Mice Resulted in Persistence of Neonatal Testicular Germ Cells (Gonocytes). Sex. Develop.
Tien MY, Abeydeera SA, Cho H-J, Sarila G, Catubig A, Burton E, Hutson J, Li R (2019) Does the Apoptosis Pathway Play a Critical Role in Gonocyte Transformation? J Pediatr Surg. 2020; 55(9):1947-1951.
Li R, Azzollini D, Shen R, Thorup J, Clasen-Linde E, Cortes D, Hutson JM. (2019) Postnatal germ cell development during first 18 months of life in testes from boys with non-syndromic cryptorchidism and complete or partial androgen insensitivity syndrome. J Pediatr Surg; 4(8):1654-1659.
Loebenstein, M., Hutson, J., and Li R. (2018) Gonocyte transformation in a congenitally cryptorchid rat is normal and may be similar to the situation reported in human acquired cryptorchidism. J Pediatr Surg 53 (9), 1770-177.
Li R, Vannitamby A, Yue SSK, Handelsman D, Hutson J. (2017), Mouse minipuberty coincides with gonocyte transformation into spermatogonial stem cells: a model for human “minipuberty”. Reprod. Fert. and Dev. Nov. 29: 2430-2436.
Li R, Vannitamby A, Zhang JG, Fehmel EL, Southwell BR, Hutson J. (2015) Oct4-GFP expression during transformation of gonocytes into spermatogonial stem cells in the perinatal mouse testis. J Pediatr Surg. 50: 2084-2089.
Li R, Vannitamby A, Meijer J, Southwell B, Hutson J. (2015) Postnatal Germ Cell Development during Mini-Puberty in the Mouse Does Not Require Androgen Receptor: Implications for Managing Cryptorchidism. J Urol;193: 1361-1367
Yue SS, Hutson JM, Li R. (2015) Gene expression during gonocyte transformation into spermatogonial stem cells is not androgen dependent. J Pediatr Surg. 50: 2090-2093.
Perera N, Szarek M, Vannitamby A., Vikraman J, Huan G, Durston A, Hutson J, and Li R. (2018) An immunohistochemical analysis of the effects of androgen receptor knock out on gubernacular differentiation in the mouse. J Pediatr Surg, 53 (9):1776-1780
Su S, Szarek M, Vooght A, Hutson J, Li R. (2014) Gonocyte transformation to spermatogonial stem cells occurs earlier in patients with undervirilisation syndromes. J Pediatr Surg. 49: 323-327
Li R, Thorup J, Sun C, Cortes D, Southwell B, Hutson J. (2014) Immunofluorescence analysis of testicular biopsies with germ cell and Sertoli cell markers shows significant MVH negative germ cell depletion with older age of orchidopexy. J. Urology, 191:458-464
Li R, Hartley L, and Robb L, (2001) Cloning of Rat interleukin 11 and interleukin 11 receptor alpha chain and analysis of their expression in rat uterus in the peri-implantation period. Reproduction, 122, 593-600.
Robb R, Li R, Hartley L, Nandurkar HH, Koentgen F, Begley CG. (1998) Infertility in female mice lacking the receptor for interlukin 11 is due to a defective uterine response to implantation. Nature Medicine, 4, 303-308.
Lyons I, Parsons LM, Hartley L, Li R, Andrews J, Robb L and Harvey RP. (1995) Myogenic and morphogenetic defects in the heart tubes of murine embryos lacking the homeobox gene Nkx2.5. Gene and Development, 9, 1654-1666
Li R, Cameron, AWN, Batt PA and Trounson AO. (1990) Maximum survival of frozen goat embryos is attained at the expanded, hatching and hatched blastocysts stages of development. Reprod, Fertil & Dev. 2, 350 354.
MCRI theme funding (CIA) for July 2011-June 2012 ($18,700): Postnatal germ cell development: the key to preventing infertility and cancer in cryptorchidism
National Health and Medical Research Council project fund APP1049014 (CID) for 2013-2015 ($700,377.60): germ cell development in the postnatal testis: the key to early surgery to prevent infertility and malignancy in cryptorchidism
National Health and Medical Research Council project fund APP1127109 (CIB) for 2013-2015 ($813,739.00): Postnatal germ cells are controlled by FSH during 'minipuberty' at 3-6 months of age, and deranged by cryptorchidism to cause seminoma and infertility
National Health and Medical Research Council project fund APP1144752 (CIB); for 2018-2020 ($743,8748):The cause of undescended testis and inguinal hernia.